• Immunohistochemistry analysis of paraffin-embedded mouse intestin using GIP Rabbit polyclonal antibody (STJ27986) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse small intestine, using GIP antibody (STJ27986) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GIP antibody (22-153) (STJ27986)

SKU:
STJ27986

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GIP (22-153) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GIP
Gene ID: 2695
Uniprot ID: GIP_HUMAN
Immunogen Region: 22-153
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-153 of human GIP (NP_004114.1).
Immunogen Sequence: EKKEGHFSALPSLPVGSHAK VSSPQPRGPRYAEGTFISDY SIAMDKIHQQDFVNWLLAQK GKKNDWKHNITQREARALEL ASQANRKEEEAVEPQSSPAK NPSDEDLLRDLLIQELLACL LDQTNLCRLRSR
Function Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
Protein Name Gastric Inhibitory Polypeptide
Gip
Glucose-Dependent Insulinotropic Polypeptide
Incretin Hormone
Database Links Reactome: R-HSA-400511
Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Secreted
Alternative Antibody Names Anti-Gastric Inhibitory Polypeptide antibody
Anti-Gip antibody
Anti-Glucose-Dependent Insulinotropic Polypeptide antibody
Anti-Incretin Hormone antibody
Anti-GIP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance