Anti-GGA3 antibody (VHS domain) (STJ13101144)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the VHS domain of human GGA3 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse, chicken.
General Information
| Short Description | Sheep polyclonal anti-GGA3 (VHS domain) for use in IHC and WB in Mouse, Rat and Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Sheep |
| Reactivity | Mouse/Rat/Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A concentration of 10-50 ยตg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Gga3 |
| Gene ID | 260302 |
| Uniprot ID | GGA3_MOUSE |
| Immunogen | A synthetic peptide from the VHS domain of human GGA3 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse, chicken. |
| Immunogen Region | VHS domain |
| Specificity | Specific for GGA3. |
Additional Info
| Function | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Involved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane. |
| Protein Name | Adp-Ribosylation Factor-Binding Protein Gga3Golgi-Localized - Gamma Ear-Containing - Arf-Binding Protein 3 |
| Database Links | Reactome: R-MMU-8875656 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembranePeripheral Membrane ProteinEndosome MembraneEarly Endosome MembraneRecycling Endosome Membrane |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor-Binding Protein Gga3 antibodyAnti-Golgi-Localized - Gamma Ear-Containing - Arf-Binding Protein 3 antibodyAnti-Gga3 antibodyAnti-Kiaa0154 antibody |
Information sourced from Uniprot.org