| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-GGA3 (VHS domain) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Gga3 |
| Gene ID: |
260302 |
| Uniprot ID: |
GGA3_MOUSE |
| Immunogen Region: |
VHS domain |
| Specificity: |
Specific for GGA3. |
| Immunogen: |
A synthetic peptide from the VHS domain of human GGA3 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat, mouse, chicken. |
| Function | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Involved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane. |
| Protein Name | Adp-Ribosylation Factor-Binding Protein Gga3Golgi-Localized - Gamma Ear-Containing - Arf-Binding Protein 3 |
| Database Links | Reactome: R-MMU-8875656 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembranePeripheral Membrane ProteinEndosome MembraneEarly Endosome MembraneRecycling Endosome Membrane |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor-Binding Protein Gga3 antibodyAnti-Golgi-Localized - Gamma Ear-Containing - Arf-Binding Protein 3 antibodyAnti-Gga3 antibodyAnti-Kiaa0154 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
