| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-GGA3 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-5µg/mlELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
GGA3 |
| Gene ID: |
23163 |
| Uniprot ID: |
GGA3_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_619525.1; NP_054720.1 |
| Specificity: |
This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720) |
| Immunogen Sequence: |
EQLSTEVGEVDQ |
| Post Translational Modifications | Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA3 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Ubiquitinated. Proteolytically cleaved during apoptosis by CASP3. |
| Function | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane. |
| Protein Name | Adp-Ribosylation Factor-Binding Protein Gga3Golgi-Localized - Gamma Ear-Containing - Arf-Binding Protein 3 |
| Database Links | Reactome: R-HSA-8854214Reactome: R-HSA-8875656Reactome: R-HSA-977225 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembranePeripheral Membrane ProteinEndosome MembraneEarly Endosome MembraneRecycling Endosome Membrane |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor-Binding Protein Gga3 antibodyAnti-Golgi-Localized - Gamma Ear-Containing - Arf-Binding Protein 3 antibodyAnti-GGA3 antibodyAnti-KIAA0154 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
