Anti-GFP-Tag antibody [SMM15111] {Affinity Gel} (STJ11109841)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationAffinity Gel
IsotypeIgG2bk
ImmunogenThis information is considered to be commercially sensitive.
General Information
| Short Description | Mouse monoclonal GFP-Tag antibody for use in IP in species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IP |
| Host | Mouse |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SMM15111 |
| Isotype | IgG2bk |
| Conjugation | Affinity Gel |
| Purification | Affinity purification |
| Dilution Range | IP, 20ul antibody (bead slurry) for 200 Mu g-400 Mu g extracts of whole cells |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | This information is considered to be commercially sensitive. |
Additional Info
| Background | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes/substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells. |
Information sourced from Uniprot.org