| Host: | Goat |
| Applications: | WB/IF/IHC-F |
| Reactivity: | GFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-GFP for use in WB, IF and IHC-F in GFP samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | DyLight®680 |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | Epitope affinity purified |
| Concentration: | 2.5 mg/mL |
| Dilution Range: | WB:1:500-1:5, 000IF:1:50-1:1, 000IHC-F:1:50-1:1, 000 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein. |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: | MVSKGEELFTGVVPILVELD VSKGEELFTGVVPILVELDG SKGEELFTGVVPILVELDGD KGEELFTGVVPILVELDGDV GEELFTGVVPILVELDGDVN EELFTGVVPILVELDGDVNG ELFTGVVPILVELDGDVNGH LFTGVVPILVELDGDVNGHK FTGVVPILVELDGDVNGHKF TGVVPILVELDGDVNGHKFS GVVPILVELDGDVNGHKFSV VVPILVELDGDVNGHKFSV |
| Background | Goat polyclonal antibody to GFP (green fluorescent protein) conjugated to DyLight® 680. GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression. |
Information sourced from Uniprot.org