• Western blot analysis using Gfi-1 monoclonal antibody against HEK293 (1) and GFI1-hIgGFc transfected HEK293 (2) cell lysate.

Anti-GFI1 antibody [5D7] (STJ98107)

SKU:
STJ98107

Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Zinc finger protein Gfi-1 is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5D7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFI1
Gene ID: 2672
Uniprot ID: GFI1_HUMAN
Specificity: Gfi-1 Monoclonal Antibody detects endogenous levels of Gfi-1 protein.
Immunogen: Purified recombinant fragment of human Gfi-1 expressed in E. Coli.
Function Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCACATGCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Inhibits SPI1 transcriptional activity at macrophage-specific genes, repressing macrophage differentiation of myeloid progenitor cells and promoting granulocyte commitment. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.
Protein Name Zinc Finger Protein Gfi-1
Growth Factor Independent Protein 1
Zinc Finger Protein 163
Database Links Reactome: R-HSA-9616222
Cellular Localisation Nucleus
Colocalizes With Pias3 And Runx1t1 In Nuclear Dots
Alternative Antibody Names Anti-Zinc Finger Protein Gfi-1 antibody
Anti-Growth Factor Independent Protein 1 antibody
Anti-Zinc Finger Protein 163 antibody
Anti-GFI1 antibody
Anti-ZNF163 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance