| Short Description : | Rabbit polyclonal GEN1 (669-908) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GEN1 |
| Gene ID: | 348654 |
| Uniprot ID: | GEN_HUMAN |
| Immunogen Region: | 669-908 |
| Immunogen Sequence: | LQDLPLKERIFTKLSYPQDN LQPDVNLKTLSILSVKESCI ANSGSDCTSHLSKDLPGIPL QNESRDSKILKGDQLLQEDY KVNTSVPYSVSNTVVKTCNV RPPNTALDHSRKVDMQTTRK ILMKKSVCLDRHSSDEQSAP VFGKAKYTTQRMKHSSQKHN SSHFKESGHNKLSSPKIHIK ETEQCVRSYETAENEESCFP DSTKSSLSSLQCHKKENNSG TCLDSPLPLRQRLKLRFQS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 669-908 of human GEN1 (NP_872431.3). |
| Function | Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Also has endonuclease activity on 5'-flap and replication fork (RF) DNA substrates. |
| Protein Name | Flap Endonuclease Gen Homolog 1 |
| Database Links | Reactome: R-HSA-5693568 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Flap Endonuclease Gen Homolog 1 antibodyAnti-GEN1 antibody |
Information sourced from Uniprot.org
