Western blot analysis of various lysates using GCNT3 Rabbit polyclonal antibody (STJ115175) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-GCNT3 antibody (27-180) (STJ115175)

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STJ115175
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCNT3
Gene ID: 9245
Uniprot ID: GCNT3_HUMAN
Immunogen Region: 27-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 27-180 of human GCNT3 (NP_004742.1).
Immunogen Sequence: LKLSFRLKCDSDHLGLESRE SQSQYCRNILYNFLKLPAKR SINCSGVTRGDQEAVLQAIL NNLEVKKKREPFTDTHYLSL TRDCEHFKAERKFIQFPLSK EEVEFPIAYSMVIHEKIENF ERLLRAVYAPQNIYCVHVDE KSPETFKEAVKAII
Tissue Specificity Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
Post Translational Modifications N-glycosylated.
Function Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Protein Name Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -6-N-Acetylglucosaminyltransferase 3
C2gnt-Mucin Type
C2gnt-M
Hc2gnt-M
Core 2/Core 4 Beta-1 -6-N-Acetylglucosaminyltransferase
C2/4gnt
Database Links Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -6-N-Acetylglucosaminyltransferase 3 antibody
Anti-C2gnt-Mucin Type antibody
Anti-C2gnt-M antibody
Anti-Hc2gnt-M antibody
Anti-Core 2/Core 4 Beta-1 -6-N-Acetylglucosaminyltransferase antibody
Anti-C2/4gnt antibody
Anti-GCNT3 antibody

Information sourced from Uniprot.org

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