| Tissue Specificity | Ubiquitously expressed. Expressed in skeletal muscules, ovary and testis. |
| Function | Ribosome collision sensor that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Directly binds to the ribosome and acts as a sentinel for colliding ribosomes: activated following ribosome stalling and promotes recruitment of RNF14, which directly ubiquitinates EEF1A1/eEF1A, leading to its degradation. In addition to EEF1A1/eEF1A, the RNF14-RNF25 translation quality control pathway mediates degradation of ETF1/eRF1 and ubiquitination of ribosomal protein. GCN1 also acts as a positive activator of the integrated stress response (ISR) by mediating activation of EIF2AK4/GCN2 in response to amino acid starvation. Interaction with EIF2AK4/GCN2 on translating ribosomes stimulates EIF2AK4/GCN2 kinase activity, leading to phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1). EIF2S1/eIF-2-alpha phosphorylation converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. |
| Protein Name | Stalled Ribosome Sensor Gcn1Gcn1 Eif-2-Alpha Kinase Activator HomologGcn1-Like Protein 1General Control Of Amino-Acid Synthesis 1-Like Protein 1Translational Activator Gcn1Hsgcn1 |
| Database Links | Reactome: R-HSA-9633012 |
| Cellular Localisation | CytoplasmAssociates With Ribosomes In Undifferentiated Neuroblastoma Cells And Increases After Neuronal Differentiation |
| Alternative Antibody Names | Anti-Stalled Ribosome Sensor Gcn1 antibodyAnti-Gcn1 Eif-2-Alpha Kinase Activator Homolog antibodyAnti-Gcn1-Like Protein 1 antibodyAnti-General Control Of Amino-Acid Synthesis 1-Like Protein 1 antibodyAnti-Translational Activator Gcn1 antibodyAnti-Hsgcn1 antibodyAnti-GCN1 antibodyAnti-GCN1L1 antibodyAnti-KIAA0219 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ11100727) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89665/358162/STJ11100727_1__67701.1713122464.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ11100727) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30S.")
![Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ11100727) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89665/358162/STJ11100727_1__67701.1713122464.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T cells, using [KO Validated] GCN1L1 Rabbit polyclonal antibody (STJ11100727) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30S.")
