• Immunofluorescence analysis of rat liver cells using GCGR Rabbit polyclonal antibody (STJ118008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GCGR antibody (STJ118008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using GCGR antibody (STJ118008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GCGR antibody (50-150) (STJ118008)

SKU:
STJ118008

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCGR (50-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCGR
Gene ID: 2642
Uniprot ID: GLR_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GCGR (NP_000151.1).
Immunogen Sequence: LPPPTELVCNRTFDKYSCWP DTPANTTANISCPWYLPWHH KVQHRFVFKRCGPDGQWVRG PRGQPWRDASQCQMDGEEIE VQKEVAKMYSSFQVMYTVGY S
Post Translational Modifications Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding.
Function G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
Protein Name Glucagon Receptor
Gl-R
Database Links Reactome: R-HSA-163359
Reactome: R-HSA-416476
Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Is Rapidly Internalized After Ligand-Binding
Alternative Antibody Names Anti-Glucagon Receptor antibody
Anti-Gl-R antibody
Anti-GCGR antibody

Information sourced from Uniprot.org

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