Anti-GCGR antibody (50-150) (STJ118008)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GCGR (50-150) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GCGR |
| Gene ID | 2642 |
| Uniprot ID | GLR_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | LPPPTELVCNRTFDKYSCWP DTPANTTANISCPWYLPWHH KVQHRFVFKRCGPDGQWVRG PRGQPWRDASQCQMDGEEIE VQKEVAKMYSSFQVMYTVGY S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GCGR (NP_000151.1). |
Additional Info
| Post Translational Modifications | Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding. |
| Function | G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system. |
| Protein Name | Glucagon ReceptorGl-R |
| Database Links | Reactome: R-HSA-163359Reactome: R-HSA-416476Reactome: R-HSA-418555Reactome: R-HSA-420092 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinIs Rapidly Internalized After Ligand-Binding |
| Alternative Antibody Names | Anti-Glucagon Receptor antibodyAnti-Gl-R antibodyAnti-GCGR antibody |
Information sourced from Uniprot.org