• Western blot analysis of extracts of various cell lines, using Vitamin D Binding protein antibody (STJ11104828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GC antibody (305-474) [S4828RM] (STJ11104828)

SKU:
STJ11104828

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Vitamin D-Binding Protein (305-474) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4828RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GC
Gene ID: 2638
Uniprot ID: VTDB_HUMAN
Immunogen Region: 305-474
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 305-474 of human Vitamin D Binding protein (NP_000574.2).
Immunogen Sequence: AMDVFVCTYFMPAAQLPELP DVELPTNKDVCDPGNTKVMD KYTFELSRRTHLPEVFLSKV LEPTLKSLGECCDVEDSTTC FNAKGPLLKKELSSFIDKGQ ELCADYSENTFTEYKKKLAE RLKAKLPDATPTELAKLVNK HSDFASNCCSINSPPLYCDS EIDAELKNIL
Tissue Specificity Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.
Post Translational Modifications Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.
Function Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Protein Name Vitamin D-Binding Protein
Dbp
Vdb
Gc Protein-Derived Macrophage Activating Factor
Gc-Maf
Gcmaf
Gc-Globulin
Group-Specific Component
Gc
Vitamin D-Binding Protein-Macrophage Activating Factor
Dbp-Maf
Database Links Reactome: R-HSA-196791
Cellular Localisation Secreted
Alternative Antibody Names Anti-Vitamin D-Binding Protein antibody
Anti-Dbp antibody
Anti-Vdb antibody
Anti-Gc Protein-Derived Macrophage Activating Factor antibody
Anti-Gc-Maf antibody
Anti-Gcmaf antibody
Anti-Gc-Globulin antibody
Anti-Group-Specific Component antibody
Anti-Gc antibody
Anti-Vitamin D-Binding Protein-Macrophage Activating Factor antibody
Anti-Dbp-Maf antibody
Anti-GC antibody

Information sourced from Uniprot.org

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