• Western blot analysis of extracts of various cell lines, using GATAD2B antibody (STJ111801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GATAD2B antibody (STJ111801). Western blot was performed from the immunoprecipitate using GATAD2B antibody (STJ111801) at a dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using GATAD2B rabbit polyclonal antibody (STJ111801) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GATAD2B in paraffin-embedded mouse pancreas using GATAD2B Rabbit polyclonal antibody (STJ111801) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug GATAD2B antibody (STJ111801). Western blot was performed from the immunoprecipitate using GATAD2B antibody (STJ111801) at a dilition of 1:1000.
  • Western blot analysis of various lysates using GATAD2B Rabbit polyclonal antibody (STJ111801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GATAD2B antibody (1-120) (STJ111801)

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STJ111801

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GATAD2B (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATAD2B
Gene ID: 57459
Uniprot ID: P66B_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GATAD2B (NP_065750.1).
Immunogen Sequence: MDRMTEDALRLNLLKRSLDP ADERDDVLAKRLKMEGHEAM ERLKMLALLKRKDLANLEVP HELPTKQDGSGVKGYEEKLN GNLRPHGDNRTAGRPGKENI NDEPVDMSARRSEPERGRLT
Tissue Specificity Widely expressed.
Function Transcriptional repressor. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A. Targets MBD3 to discrete loci in the nucleus. May play a role in synapse development.
Protein Name Transcriptional Repressor P66-Beta
Gata Zinc Finger Domain-Containing Protein 2b
P66/P68
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Cellular Localisation Nucleus Speckle
Nucleus
Chromosome
Speckled Nuclear Localization Requires Both Cr1 And Cr2 Regions
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Transcriptional Repressor P66-Beta antibody
Anti-Gata Zinc Finger Domain-Containing Protein 2b antibody
Anti-P66/P68 antibody
Anti-GATAD2B antibody
Anti-KIAA1150 antibody

Information sourced from Uniprot.org

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