• Western blot analysis of Mouse brain, using GATA3 Rabbit polyclonal antibody (STJ11104558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-GATA3 antibody (1-260) (STJ11104558)

SKU:
STJ11104558

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA3
Gene ID: 2625
Uniprot ID: GATA3_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GATA3 (NP_001002295.1).
Immunogen Sequence: MEVTADQPRWVSHHHPAVLN GQHPDTHHPGLSHSYMDAAQ YPLPEEVDVLFNIDGQGNHV PPYYGNSVRATVQRYPPTHH GSQVCRPPLLHGSLPWLDGG KALGSHHTASPWNLSPFSKT SIHHGSPGPLSVYPPASSSS LSGGHASPHLFTFPPTPPKD VSPDPSLSTPGSAGSARQDE KECLKYQVPLPDSMKLESSH SRGSMTALGGASSSTHHPIT TYPPYVPEYSSGLFPPSSL
Tissue Specificity T-cells and endothelial cells.
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Protein Name Trans-Acting T-Cell-Specific Transcription Factor Gata-3
Gata-Binding Factor 3
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-6785807
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 antibody
Anti-Gata-Binding Factor 3 antibody
Anti-GATA3 antibody

Information sourced from Uniprot.org

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