• Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAS2 antibody (STJ23749) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GAS2 antibody (STJ23749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-GAS2 antibody (1-100) (STJ23749)

SKU:
STJ23749

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GAS2 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAS2
Gene ID: 2620
Uniprot ID: GAS2_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human GAS2 (NP_005247.1).
Immunogen Sequence: MCTALSPKVRSGPGLSDMHQ YSQWLASRHEANLLPMKEDL ALWLTNLLGKEITAETFMEK LDNGALLCQLAETMQEKFKE SMDANKPTKNLPLKKIPCKT
Tissue Specificity Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
Post Translational Modifications Cleaved, during apoptosis, on a specific aspartic residue by caspases. Phosphorylated on serine residues during the G0-G1 transition phase.
Function May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
Protein Name Growth Arrest-Specific Protein 2
Gas-2
Database Links Reactome: R-HSA-264870
Cellular Localisation Cytoplasm
Cytoskeleton
Stress Fiber
Membrane
Peripheral Membrane Protein
Component Of The Microfilament System
Colocalizes With Actin Fibers At The Cell Border And Along The Stress Fibers In Growth-Arrested Fibroblasts
Mainly Membrane-Associated
When Hyperphosphorylated
Accumulates At Membrane Ruffles
Alternative Antibody Names Anti-Growth Arrest-Specific Protein 2 antibody
Anti-Gas-2 antibody
Anti-GAS2 antibody

Information sourced from Uniprot.org

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