• Confocal immunofluorescence analysis of NIH-3T3 cells using GARS Polyclonal Antibody (STJ27388) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using GARS Rabbit polyclonal antibody (STJ27388) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GARS Rabbit polyclonal antibody (STJ27388) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using GARS Rabbit polyclonal antibody (STJ27388) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using GARS Rabbit polyclonal antibody (STJ27388) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using GARS Rabbit polyclonal antibody (STJ27388) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from wild type (WT) and GARS knockout (KO) 293T cells, using GARS Rabbit polyclonal antibody (STJ27388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates, using GARS Rabbit polyclonal antibody (STJ27388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GARS antibody (60-370) (STJ27388)

SKU:
STJ27388

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GARS (60-370) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GARS1
Gene ID: 2617
Uniprot ID: GARS_HUMAN
Immunogen Region: 60-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2).
Immunogen Sequence: AEEVLAPLRLAVRQQGDLVR KLKEDKAPQVDVDKAVAELK ARKRVLEAKELALQPKDDIV DRAKMEDTLKRRFFYDQAFA IYGGVSGLYDFGPVGCALKN NIIQTWRQHFIQEEQILEID CTMLTPEPVLKTSGHVDKFA DFMVKDVKNGECFRADHLLK AHLQKLMSDKKCSVEKKSEM ESVLAQLDNYGQQELADLFV NYNVKSPITGNDLSPPVSFN LMFKTFIGPGGNMPGYLRP
Tissue Specificity Widely expressed, including in brain and spinal cord. Isoform 2: Expressed in brain, spinal cord, muscle, heart and spleen. Isoform 1: Expressed in brain, spinal cord, muscle, heart, spleen and liver.
Function Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
Protein Name Glycine--Trna Ligase
Diadenosine Tetraphosphate Synthetase
Ap4a Synthetase
Glycyl-Trna Synthetase
Glyrs
Glycyl-Trna Synthetase 1
Database Links Reactome: R-HSA-379716
Reactome: R-HSA-379726
Cellular Localisation Cytoplasm
Cell Projection
Axon
Secreted
Extracellular Exosome
In Transfected Cos7 Cells
Not Detected In Mitochondria
Nor In Golgi Apparatus
Secreted By Motor Neuron
Possibly Through The Exosome Pathway
Isoform 1: Mitochondrion
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Glycine--Trna Ligase antibody
Anti-Diadenosine Tetraphosphate Synthetase antibody
Anti-Ap4a Synthetase antibody
Anti-Glycyl-Trna Synthetase antibody
Anti-Glyrs antibody
Anti-Glycyl-Trna Synthetase 1 antibody
Anti-GARS1 antibody
Anti-GARS antibody

Information sourced from Uniprot.org

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