| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GAPVD1 |
| Gene ID: |
26130 |
| Uniprot ID: |
GAPD1_HUMAN |
| Immunogen Region: |
1-260 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GAPVD1 (NP_056450.2). |
| Immunogen Sequence: |
MVKLDIHTLAHHLKQERLYV NSEKQLIQRLNADVLKTAEK LYRTAWIAKQQRINLDRLII TSAEASPAECCQHAKILEDT QFVDGYKQLGFQETAYGEFL SRLRENPRLIASSLVAGEKL NQENTQSVIYTVFTSLYGNC IMQEDESYLLQVLRYLIEFE LKESDNPRRLLRRGTCAFSI LFKLFSEGLFSAKLFLTATL HEPIMQLLVEDEDHLETDPN KLIERFSPSQQEKLFGEKG |
| Tissue Specificity | Expressed in erythrocytes (at protein level). |
| Function | Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras. |
| Protein Name | Gtpase-Activating Protein And Vps9 Domain-Containing Protein 1Gapex-5Rab5-Activating Protein 6 |
| Database Links | Reactome: R-HSA-8856828Reactome: R-HSA-8876198 |
| Cellular Localisation | MembranePeripheral Membrane ProteinEndosomeRecruited To The Plasma Membrane By Trip10/Cip4 In Response To Insulin |
| Alternative Antibody Names | Anti-Gtpase-Activating Protein And Vps9 Domain-Containing Protein 1 antibodyAnti-Gapex-5 antibodyAnti-Rab5-Activating Protein 6 antibodyAnti-GAPVD1 antibodyAnti-GAPEX5 antibodyAnti-KIAA1521 antibodyAnti-RAP6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
