Anti-GAPDH antibody (240 aa C-Term) {DyLight®488} (STJ140265)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationDyLight®488
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
General Information
| Short Description | Goat polyclonal GAPDH (240 aa C-Term) antibody for use in WB, IF and IHC-F in human, rat, mouse, zebrafish, canine and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IHC-F |
| Host | Goat |
| Reactivity | Human/Rat/Mouse/Zebrafish/Canine/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | DyLight®488 |
| Concentration | 2 mg/mL |
| Purification | Epitope affinity purified |
| Dilution Range | WB 1:500-1:5000IHC-F 1:200-1:1000IF 1:50-1:250 |
| Formulation | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | GAPDH |
| Gene ID | 2597 |
| Uniprot ID | G3P_HUMAN |
| Immunogen | Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli. |
| Immunogen Region | 240 aa C-Term |
| Immunogen Sequence | SVVDLTCRLEKPAKYDDIKK VVDLTCRLEKPAKYDDIKKV VDLTCRLEKPAKYDDIKKVV DLTCRLEKPAKYDDIKKVVK LTCRLEKPAKYDDIKKVVKQ |
| Specificity | Detects a band of 37 kDa by Western blot. |
Additional Info
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. |
| Protein Name | Glyceraldehyde-3-Phosphate DehydrogenaseGapdhPeptidyl-Cysteine S-Nitrosylase Gapdh |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263 |
| Cellular Localisation | CytoplasmCytosolNucleusPerinuclear RegionMembraneCytoskeletonTranslocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1Which Contains A Nuclear Localization SignalPostnuclear And Perinuclear Regions |
| Alternative Antibody Names | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibodyAnti-Gapdh antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibodyAnti-GAPDH antibodyAnti-GAPD antibodyAnti-CDABP0047 antibodyAnti-OK antibodyAnti-SW-cl.12 antibody |
Information sourced from Uniprot.org