Anti-GADD34 antibody (380-400) {FITC} (STJ501135)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationFITC
IsotypeIgG
ImmunogenSynthetic peptide corresponding to internal sequence ofΒ amino acids 380-400Β of Human GADD34, covalently modified and coupled to a carrier protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-GADD34 (380-400) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Bovine/Human/Monkey/Panda |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | FITC |
| Concentration | 0.58 Β΅g/Β΅l |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:800-1:1, 000ELISA: 1:800-1:1, 000IP: 1:200IHC: 1:50-1:150 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20Β°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PPP1R15A |
| Gene ID | 23645 |
| Uniprot ID | PR15A_HUMAN |
| Immunogen | Synthetic peptide corresponding to internal sequence ofΒ amino acids 380-400Β of Human GADD34, covalently modified and coupled to a carrier protein. |
| Immunogen Region | 380-400 |
Additional Info
| Post Translational Modifications | Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN.which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2. Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association. |
| Function | Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Acts also a viral restriction factor by attenuating HIV-1 replication. Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 15aGrowth Arrest And Dna Damage-Inducible Protein Gadd34Myeloid Differentiation Primary Response Protein Myd116 Homolog |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-9648895 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideMitochondrion Outer MembraneAssociates With Membranes Via An N-Terminal Amphipathic Intramembrane Region |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 15a antibodyAnti-Growth Arrest And Dna Damage-Inducible Protein Gadd34 antibodyAnti-Myeloid Differentiation Primary Response Protein Myd116 Homolog antibodyAnti-PPP1R15A antibodyAnti-GADD34 antibody |
Information sourced from Uniprot.org