• STJ70691 (0. 5µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70691 (2. 5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Anti-GAD2/GAD65 antibody (Internal) (STJ70691)

SKU:
STJ70691-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GAD2/GAD65 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 h
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GAD2
Gene ID: 2572
Uniprot ID: DCE2_HUMAN
Immunogen Region: Internal
Accession Number: NP_000809.1; NP_001127838.1
Specificity: This antibody is expected to recognise both reported variants.
Immunogen Sequence: TLEDNEERMSRLSK
Function Catalyzes the production of GABA.
Protein Name Glutamate Decarboxylase 2
65 Kda Glutamic Acid Decarboxylase
Gad-65
Glutamate Decarboxylase 65 Kda Isoform
Database Links Reactome: R-HSA-888568
Reactome: R-HSA-888590
Reactome: R-HSA-9022927
Cellular Localisation Cytoplasm
Cytosol
Cytoplasmic Vesicle
Presynaptic Cell Membrane
Lipid-Anchor
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Associated To Cytoplasmic Vesicles
In Neurons
Cytosolic Leaflet Of Golgi Membranes And Presynaptic Clusters
Alternative Antibody Names Anti-Glutamate Decarboxylase 2 antibody
Anti-65 Kda Glutamic Acid Decarboxylase antibody
Anti-Gad-65 antibody
Anti-Glutamate Decarboxylase 65 Kda Isoform antibody
Anti-GAD2 antibody
Anti-GAD65 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance