| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GAD2/GAD65 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 h |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | GAD2 |
| Gene ID: | 2572 |
| Uniprot ID: | DCE2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000809.1; NP_001127838.1 |
| Specificity: | This antibody is expected to recognise both reported variants. |
| Immunogen Sequence: | TLEDNEERMSRLSK |
| Function | Catalyzes the production of GABA. |
| Protein Name | Glutamate Decarboxylase 2 65 Kda Glutamic Acid Decarboxylase Gad-65 Glutamate Decarboxylase 65 Kda Isoform |
| Database Links | Reactome: R-HSA-888568 Reactome: R-HSA-888590 Reactome: R-HSA-9022927 |
| Cellular Localisation | Cytoplasm Cytosol Cytoplasmic Vesicle Presynaptic Cell Membrane Lipid-Anchor Golgi Apparatus Membrane Peripheral Membrane Protein Cytoplasmic Side Associated To Cytoplasmic Vesicles In Neurons Cytosolic Leaflet Of Golgi Membranes And Presynaptic Clusters |
| Alternative Antibody Names | Anti-Glutamate Decarboxylase 2 antibody Anti-65 Kda Glutamic Acid Decarboxylase antibody Anti-Gad-65 antibody Anti-Glutamate Decarboxylase 65 Kda Isoform antibody Anti-GAD2 antibody Anti-GAD65 antibody |
Information sourced from Uniprot.org