• Western blot analysis of extracts of rat skeletal muscle, using GABRA5 antibody (STJ115919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GABRA5 antibody (32-259) (STJ115919)

SKU:
STJ115919

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABRA5
Gene ID: 2558
Uniprot ID: GBRA5_HUMAN
Immunogen Region: 32-259
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 32-259 of human GABRA5 (NP_000801.1).
Immunogen Sequence: QMPTSSVKDETNDNITIFTR ILDGLLDGYDNRLRPGLGER ITQVRTDIYVTSFGPVSDTE MEYTIDVFFRQSWKDERLRF KGPMQRLPLNNLLASKIWTP DTFFHNGKKSIAHNMTTPNK LLRLEDDGTLLYTMRLTISA ECPMQLEDFPMDAHACPLKF GSYAYPNSEVVYVWTNGSTK SVVVAEDGSRLNQYHLMGQT VGTENISTSTGEYTIMTAHF HLKRKIGY
Function Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-5/GABRA5 subunits are mainly extrasynaptic and contribute to the tonic GABAergic inhibition in the hippocampus. Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes.
Protein Name Gamma-Aminobutyric Acid Receptor Subunit Alpha-5
Gaba(A Receptor Subunit Alpha-5
Gabaar Subunit Alpha-5
Database Links Reactome: R-HSA-977443
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-5 antibody
Anti-Gaba(A Receptor Subunit Alpha-5 antibody
Anti-Gabaar Subunit Alpha-5 antibody
Anti-GABRA5 antibody

Information sourced from Uniprot.org

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