• Western blot analysis of various lysates using GABRA3 Rabbit polyclonal antibody (STJ113241) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GABRA3 antibody (29-276) (STJ113241)

SKU:
STJ113241

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABRA3
Gene ID: 2556
Uniprot ID: GBRA3_HUMAN
Immunogen Region: 29-276
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 29-276 of human GABRA3 (NP_000799.1).
Immunogen Sequence: QGESRRQEPGDFVKQDIGGL SPKHAPDIPDDSTDNITIFT RILDRLLDGYDNRLRPGLGD AVTEVKTDIYVTSFGPVSDT DMEYTIDVFFRQTWHDERLK FDGPMKILPLNNLLASKIWT PDTFFHNGKKSVAHNMTTPN KLLRLVDNGTLLYTMRLTIH AECPMHLEDFPMDVHACPLK FGSYAYTTAEVVYSWTLGKN KSVEVAQDGSRLNQYDLLGH VVGTEIIRSSTGEYVVMTT
Function Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission.
Protein Name Gamma-Aminobutyric Acid Receptor Subunit Alpha-3
Gaba(A Receptor Subunit Alpha-3
Gabaar Subunit Alpha-3
Database Links Reactome: R-HSA-977443
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-3 antibody
Anti-Gaba(A Receptor Subunit Alpha-3 antibody
Anti-Gabaar Subunit Alpha-3 antibody
Anti-GABRA3 antibody

Information sourced from Uniprot.org

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