• Immunofluorescence analysis of paraffin-embedded Human stomach using G6PD Rabbit polyclonal antibody (STJ115918) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of NIH/3T3 cells using G6PD Rabbit polyclonal antibody (STJ115918) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from C6 cells using G6PD Rabbit polyclonal antibody (STJ115918) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:30s.
  • Western blot analysis of lysates from wild type (WT) and G6PD knockdown (KD) HeLa cells using G6PD Rabbit polyclonal antibody (STJ115918) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-G6PD antibody (415-515) (STJ115918)

SKU:
STJ115918

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-G6PD (415-515) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: G6PD
Gene ID: 2539
Uniprot ID: G6PD_HUMAN
Immunogen Region: 415-515
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 415-515 of human G6PD (NP_001035810.1).
Immunogen Sequence: PEESELDLTYGNRYKNVKLP DAYERLILDVFCGSQMHFVR SDELREAWRIFTPLLHQIEL EKPKPIPYIYGSRGPTEADE LMKRVGFQYEGTYKWVNPHK L
Tissue Specificity Isoform Long is found in lymphoblasts, granulocytes and sperm.
Post Translational Modifications Acetylated by ELP3 at Lys-403.acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403.deacetylation stimulates its enzyme activity.
Function Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Protein Name Glucose-6-Phosphate 1-Dehydrogenase
G6pd
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Glucose-6-Phosphate 1-Dehydrogenase antibody
Anti-G6pd antibody
Anti-G6PD antibody

Information sourced from Uniprot.org

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