| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | G6pc1 |
| Gene ID: | 14377 |
| Uniprot ID: | G6PC1_MOUSE |
| Immunogen Region: | 1-112 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of mouse G6PC (NP_032087.2). |
| Immunogen Sequence: | MEEGMNILHDFGIQSTRYLQ VNYQDSQDWFILVSVIADLR NAFYVLFPIWFHLKETVGIN LLWVAVVGDWFNLVFKWILF GQRPYWWVLDTDYYSNSSVP IIKQFPVTCETG |
| Tissue Specificity | Liver and kidney. |
| Function | Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. |
| Protein Name | Glucose-6-Phosphatase Catalytic Subunit 1Glucose-6-PhosphataseG-6-PaseG6pase |
| Database Links | Reactome: R-MMU-70263 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Glucose-6-Phosphatase Catalytic Subunit 1 antibodyAnti-Glucose-6-Phosphatase antibodyAnti-G-6-Pase antibodyAnti-G6pase antibodyAnti-G6pc1 antibodyAnti-G6pc antibodyAnti-G6pt antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178266/370594/STJ11105622_1__58411.1713136541.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MRPL53 knockdown (KD) HeLa cells using [KD Validated] MRPL53 Rabbit polyclonal antibody (STJ11105622) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
