| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FZD4 (37-222) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FZD4 |
| Gene ID: |
8322 |
| Uniprot ID: |
FZD4_HUMAN |
| Immunogen Region: |
37-222 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 37-222 of human FZD4 (NP_036325.2). |
| Immunogen Sequence: |
FGDEEERRCDPIRISMCQNL GYNVTKMPNLVGHELQTDAE LQLTTFTPLIQYGCSSQLQF FLCSVYVPMCTEKINIPIGP CGGMCLSVKRRCEPVLKEFG FAWPESLNCSKFPPQNDHNH MCMEGPGDEEVPLPHKTPIQ PGEECHSVGTNSDQYIWVKR SLNCVLKCGYDAGLYSRSAK EFTDIW |
| Tissue Specificity | Almost ubiquitous. Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver. |
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
| Protein Name | Frizzled-4Fz-4Hfz4Fze4Cd Antigen Cd344 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4086398Reactome: R-HSA-4608870Reactome: R-HSA-4641263Reactome: R-HSA-5099900Reactome: R-HSA-5340588Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Frizzled-4 antibodyAnti-Fz-4 antibodyAnti-Hfz4 antibodyAnti-Fze4 antibodyAnti-Cd Antigen Cd344 antibodyAnti-FZD4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
