| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FZD3 (23-205) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FZD3 |
| Gene ID: |
7976 |
| Uniprot ID: |
FZD3_HUMAN |
| Immunogen Region: |
23-205 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 23-205 of human FZD3 (NP_059108.1). |
| Immunogen Sequence: |
HSLFSCEPITLRMCQDLPYN TTFMPNLLNHYDQQTAALAM EPFHPMVNLDCSRDFRPFLC ALYAPICMEYGRVTLPCRRL CQRAYSECSKLMEMFGVPWP EDMECSRFPDCDEPYPRLVD LNLAGEPTEGAPVAVQRDYG FWCPRELKIDPDLGYSFLHV RDCSPPCPNMYFRREELSFA RYF |
| Tissue Specificity | Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis. |
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in controlling early axon growth and guidance processes necessary for the formation of a subset of central and peripheral major fiber tracts. Required for the development of major fiber tracts in the central nervous system, including: the anterior commissure, the corpus callosum, the thalamocortical, corticothalamic and nigrostriatal tracts, the corticospinal tract, the fasciculus retroflexus, the mammillothalamic tract, the medial lemniscus, and ascending fiber tracts from the spinal cord to the brain. In the peripheral nervous system, controls axon growth in distinct populations of cranial and spinal motor neurons, including the facial branchimotor nerve, the hypoglossal nerve, the phrenic nerve, and motor nerves innervating dorsal limbs. Involved in the migration of cranial neural crest cells. May also be implicated in the transmission of sensory information from the trunk and limbs to the brain. Controls commissural sensory axons guidance after midline crossing along the anterior-posterior axis in the developing spinal cord in a Wnt-dependent signaling pathway. Together with FZD6, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle in a beta-catenin-dependent manner. |
| Protein Name | Frizzled-3Fz-3Hfz3 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4086398Reactome: R-HSA-4086400Reactome: R-HSA-4608870 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCell MembraneCell SurfaceApical Cell MembraneColocalizes With Fzd6 At The Apical Face Of The Cell |
| Alternative Antibody Names | Anti-Frizzled-3 antibodyAnti-Fz-3 antibodyAnti-Hfz3 antibodyAnti-FZD3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
