| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-FYN (240-290) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.55-0.75 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | FYN |
| Gene ID: | 2534 |
| Uniprot ID: | FYN_HUMAN |
| Immunogen Region: | 240-290 |
| Immunogen: | Synthetic peptide taken within amino acid region 240-290 on human Fyn protein. |
| Post Translational Modifications | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Phosphorylated at tyrosine residues, which can be enhanced by NTN1. Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location. |
| Function | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Involved in reelin signaling by mediating phosphorylation of DAB1 following reelin (RELN)-binding to its receptor. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling. Can also promote CD244-mediated NK cell activation. |
| Protein Name | Tyrosine-Protein Kinase Fyn Proto-Oncogene Syn Proto-Oncogene C-Fyn Src-Like Kinase Slk P59-Fyn |
| Database Links | Reactome: R-HSA-114604 Reactome: R-HSA-1227986 Reactome: R-HSA-1257604 Reactome: R-HSA-1433557 Reactome: R-HSA-1433559 Reactome: R-HSA-164944 Reactome: R-HSA-202733 Reactome: R-HSA-2029481 Reactome: R-HSA-210990 Reactome: R-HSA-2219530 Reactome: R-HSA-2424491 Reactome: R-HSA-2682334 Reactome: R-HSA-2730905 Reactome: R-HSA-373753 Reactome: R-HSA-375165 Reactome: R-HSA-389356 Reactome: R-HSA-389357 Reactome: R-HSA-389359 Reactome: R-HSA-389513 Reactome: R-HSA-3928662 Reactome: R-HSA-3928663 Reactome: R-HSA-3928664 Reactome: R-HSA-3928665 Reactome: R-HSA-399954 Reactome: R-HSA-399955 Reactome: R-HSA-399956 Reactome: R-HSA-418885 P06241-1 Reactome: R-HSA-4420097 Reactome: R-HSA-5621480 Reactome: R-HSA-5621575 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-75892 Reactome: R-HSA-8866376 Reactome: R-HSA-9032500 P06241-1 Reactome: R-HSA-9032759 P06241-1 Reactome: R-HSA-912631 Reactome: R-HSA-9664323 Reactome: R-HSA-9664422 Reactome: R-HSA-9670439 Reactome: R-HSA-9680350 Reactome: R-HSA-9706374 Reactome: R-HSA-983695 |
| Cellular Localisation | Cytoplasm Nucleus Cell Membrane Perikaryon Present And Active In Lipid Rafts Palmitoylation Is Crucial For Proper Trafficking |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Fyn antibody Anti-Proto-Oncogene Syn antibody Anti-Proto-Oncogene C-Fyn antibody Anti-Src-Like Kinase antibody Anti-Slk antibody Anti-P59-Fyn antibody Anti-FYN antibody |
Information sourced from Uniprot.org
