Anti-FYN antibody (240-290) {Biotin} (STJ501110)

SKU:
STJ501110-100

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Host: Rabbit
Applications: ELISA/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FYN (240-290) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.55-0.75 µg/µl
Dilution Range: WB: 1:500
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:200
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: FYN
Gene ID: 2534
Uniprot ID: FYN_HUMAN
Immunogen Region: 240-290
Immunogen: Synthetic peptide taken within amino acid region 240-290 on human Fyn protein.
Post Translational Modifications Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Phosphorylated at tyrosine residues, which can be enhanced by NTN1. Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location.
Function Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling.
Protein Name Tyrosine-Protein Kinase Fyn
Proto-Oncogene Syn
Proto-Oncogene C-Fyn
Src-Like Kinase
Slk
P59-Fyn
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1227986
Reactome: R-HSA-1257604
Reactome: R-HSA-1433557
Reactome: R-HSA-1433559
Reactome: R-HSA-164944
Reactome: R-HSA-202733
Reactome: R-HSA-2029481
Reactome: R-HSA-210990
Reactome: R-HSA-2219530
Reactome: R-HSA-2424491
Reactome: R-HSA-2682334
Reactome: R-HSA-2730905
Reactome: R-HSA-373753
Reactome: R-HSA-375165
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-389513
Reactome: R-HSA-3928662
Reactome: R-HSA-3928663
Reactome: R-HSA-3928664
Reactome: R-HSA-3928665
Reactome: R-HSA-399954
Reactome: R-HSA-399955
Reactome: R-HSA-399956
Reactome: R-HSA-418885 P06241-1
Reactome: R-HSA-4420097
Reactome: R-HSA-5621480
Reactome: R-HSA-5621575
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-75892
Reactome: R-HSA-8866376
Reactome: R-HSA-9032500 P06241-1
Reactome: R-HSA-9032759 P06241-1
Reactome: R-HSA-912631
Reactome: R-HSA-9664323
Reactome: R-HSA-9664422
Reactome: R-HSA-9670439
Reactome: R-HSA-9680350
Reactome: R-HSA-9706374
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Nucleus
Cell Membrane
Perikaryon
Present And Active In Lipid Rafts
Palmitoylation Is Crucial For Proper Trafficking
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Fyn antibody
Anti-Proto-Oncogene Syn antibody
Anti-Proto-Oncogene C-Fyn antibody
Anti-Src-Like Kinase antibody
Anti-Slk antibody
Anti-P59-Fyn antibody
Anti-FYN antibody

Information sourced from Uniprot.org

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