Anti-FUNDC1 antibody (50-155) (STJ11104646)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Fun14 Domain-Containing Protein 1 (50-155) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FUNDC1 |
| Gene ID | 139341 |
| Uniprot ID | FUND1_HUMAN |
| Immunogen Region | 50-155 |
| Immunogen Sequence | VATQIVMGGVTGWCAGFLFQ KVGKLAATAVGGGFLLLQIA SHSGYVQIDWKRVEKDVNKA KRQIKKRANKAAPEINNLIE EATEFIKQNIVISSGFVGGF LLGLAS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-155 of human FUNDC1 (NP_776155.1). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Ser-13 by CK2 and at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy. Dephosphorylation is mediated by PGAM5. Phosphorylated by ULK1 at Ser-17 which enhances FUNDC1 binding to LC3. Ubiquitinated on Lys-119. Deubiquitinated by USP19.leading to hypoxia-induced DRP1 oligomerization and GTPase activity. |
| Function | Integral mitochondrial outer-membrane protein that mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs). In turn, mediates angiogenesis and neoangiogenesis through interference with intracellular Ca(2+) communication and regulation of the vascular endothelial growth factor receptor KDR/VEGFR2 expression at both mRNA and protein levels. Also acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality and homeostasis, by interacting with and recruiting LC3 protein family to mitochondria. Mechanistically, recruits DRP1 at ER-mitochondria contact sites leading to DRP1 oligomerization and GTPase activity to facilitate mitochondrial fission during hypoxia. Additionally, plays a role in hepatic ferroptosis by interacting directly with glutathione peroxidase/GPX4 to facilitate its recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy. |
| Protein Name | Fun14 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-8934903 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Fun14 Domain-Containing Protein 1 antibodyAnti-FUNDC1 antibody |
Information sourced from Uniprot.org