• Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using FT Rabbit polyclonal antibody (STJ11104259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FT antibody (70-150) (STJ11104259)

SKU:
STJ11104259

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Host: Rabbit
Applications: WB
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FT (70-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 70-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-150 of arabidopsis thaliana FT (NP_176726.1).
Immunogen Sequence: VDPDVPSPSNPHLREYLHWL VTDIPATTGTTFGNEIVCYE NPSPTAGIHRVVFILFRQLG RQTVYAPGWRQNFNTREFAE I
Background FT, together with LFY, promotes flowering and is antagonistic with its homologous gene, TERMINAL FLOWER1 (TFL1). FT is expressed in leaves and is induced by long day treatment. Either the FT mRNA or protein is translocated to the shoot apex where it induces its own expression. Recent data suggests that FT protein acts as a long-range signal. FT is a target of CO and acts upstream of SOC1.

Information sourced from Uniprot.org

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