• STJ71477 (1µg/ml) staining of Small Intestine (Ileum) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Frizzled 4 antibody (Internal) (STJ71477)

SKU:
STJ71477-100

Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Frizzled 4 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: FZD4
Gene ID: 8322
Uniprot ID: FZD4_HUMAN
Immunogen Region: Internal
Accession Number: NP_036325.2
Immunogen Sequence: DEEERRCDPIRIS
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Protein Name Frizzled-4
Fz-4
Hfz4
Fze4
Cd Antigen Cd344
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-4086398
Reactome: R-HSA-4608870
Reactome: R-HSA-4641263
Reactome: R-HSA-5099900
Reactome: R-HSA-5340588
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Frizzled-4 antibody
Anti-Fz-4 antibody
Anti-Hfz4 antibody
Anti-Fze4 antibody
Anti-Cd Antigen Cd344 antibody
Anti-FZD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance