Host: | Goat |
Applications: | Pep-ELISA/WB |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-FOXO4/MLLT7 (Internal) is suitable for use in ELISA and Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000. WB: Approx 60-65kDa band observed in lysates of cell line K562 (calculated MW of 53.7kDa according to NP_005929.2). Recommended concentration: 1-3µg/ml. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | FOXO4 |
Gene ID: | 4303 |
Uniprot ID: | FOXO4_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_005929.2 |
Immunogen Sequence: | EAASQDRMPQDLD |
Post Translational Modifications | Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated.monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors.results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7.deubiquitination is induced by oxidative stress.enhances its interaction with USP7 and consequently, deubiquitination.increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. |
Function | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. |
Protein Name | Forkhead Box Protein O4 Fork Head Domain Transcription Factor Afx1 |
Database Links | Reactome: R-HSA-198693 Reactome: R-HSA-5674400 Reactome: R-HSA-5689880 Reactome: R-HSA-9614399 Reactome: R-HSA-9614657 Reactome: R-HSA-9615017 Reactome: R-HSA-9617629 Reactome: R-HSA-9617828 Reactome: R-HSA-9733709 |
Cellular Localisation | Cytoplasm Nucleus When Phosphorylated Translocated From Nucleus To Cytoplasm Dephosphorylation Triggers Nuclear Translocation Monoubiquitination Increases Nuclear Localization When Deubiquitinated |
Alternative Antibody Names | Anti-Forkhead Box Protein O4 antibody Anti-Fork Head Domain Transcription Factor Afx1 antibody Anti-FOXO4 antibody Anti-AFX antibody Anti-AFX1 antibody Anti-MLLT7 antibody |
Information sourced from Uniprot.org