• Western blot detection of c-Fos in Hela (TSA treated ) and Hela cell lysates using c-Fos mouse mAb (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.
  • Western blot analysis of extracts from Hela cells, untreated, 50 Mu M TSA-treated or 50 Mu M TPA-treated for 24h, using C-FOS antibody (1:500 diluted) (upper) or GAPDH antibody (1:10000 diluted) (lower).
  • Western blot detection of c-Fos in HCT116, COS7 and C6 cell lysates using c-Fos mouse mAb (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.

Anti-FOS antibody [7D6-A9-D4] (STJ99211)

SKU:
STJ99211

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Protein c-Fos is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 7D6-A9-D4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOS
Gene ID: 2353
Uniprot ID: FOS_HUMAN
Specificity: This antibody detects endogenous levels of c-Fos and does not cross-react with related proteins.
Immunogen: Purified recombinant human FOS protein fragments expressed in E.coli.
Function Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Protein Name Protein C-Fos
Cellular Oncogene Fos
Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit
G0/G1 Switch Regulatory Protein 7
Proto-Oncogene C-Fos
Transcription Factor Ap-1 Subunit C-Fos
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2871796
Reactome: R-HSA-450341
Reactome: R-HSA-6785807
Reactome: R-HSA-6796648
Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Reactome: R-HSA-9634638
Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Endoplasmic Reticulum
Cytoplasm
Cytosol
In Quiescent Cells
Present In Very Small Amounts In The Cytosol
Following Induction Of Cell Growth
First Localizes To The Endoplasmic Reticulum And Only Later To The Nucleus
Localization At The Endoplasmic Reticulum Requires Dephosphorylation At Tyr-10 And Tyr-30
Alternative Antibody Names Anti-Protein C-Fos antibody
Anti-Cellular Oncogene Fos antibody
Anti-Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit antibody
Anti-G0/G1 Switch Regulatory Protein 7 antibody
Anti-Proto-Oncogene C-Fos antibody
Anti-Transcription Factor Ap-1 Subunit C-Fos antibody
Anti-FOS antibody
Anti-G0S7 antibody

Information sourced from Uniprot.org

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