• Western blot analysis of various lysates, using PSMA/FOLH1 antibody (STJ11104752) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:2000000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of LNCaP, using PSMA/FOLH1 antibody (STJ11104752) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:2000000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FOLH1 antibody (50-150) [S4752RM] (STJ11104752)

SKU:
STJ11104752

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Glutamate Carboxypeptidase 2 (50-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4752RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOLH1
Gene ID: 2346
Uniprot ID: FOLH1_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PSMA/FOLH1 (NP_004467.1).
Immunogen Sequence: TNITPKHNMKAFLDELKAEN IKKFLYNFTQIPHLAGTEQN FQLAKQIQSQWKEFGLDSVE LAHYDVLLSYPNKTHPNYIS IINEDGNEIFNTSLFEPPPP G
Tissue Specificity Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-9 is specifically expressed in prostate cancer.
Post Translational Modifications The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis. The N-terminus is blocked.
Function Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Protein Name Glutamate Carboxypeptidase 2
Cell Growth-Inhibiting Gene 27 Protein
Folate Hydrolase 1
Folylpoly-Gamma-Glutamate Carboxypeptidase
Fgcp
Glutamate Carboxypeptidase Ii
Gcpii
Membrane Glutamate Carboxypeptidase
Mgcp
N-Acetylated-Alpha-Linked Acidic Dipeptidase I
Naaladase I
Prostate-Specific Membrane Antigen
Psm
Psma
Pteroylpoly-Gamma-Glutamate Carboxypeptidase
Database Links Reactome: R-HSA-8963693
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Isoform Psma': Cytoplasm
Alternative Antibody Names Anti-Glutamate Carboxypeptidase 2 antibody
Anti-Cell Growth-Inhibiting Gene 27 Protein antibody
Anti-Folate Hydrolase 1 antibody
Anti-Folylpoly-Gamma-Glutamate Carboxypeptidase antibody
Anti-Fgcp antibody
Anti-Glutamate Carboxypeptidase Ii antibody
Anti-Gcpii antibody
Anti-Membrane Glutamate Carboxypeptidase antibody
Anti-Mgcp antibody
Anti-N-Acetylated-Alpha-Linked Acidic Dipeptidase I antibody
Anti-Naaladase I antibody
Anti-Prostate-Specific Membrane Antigen antibody
Anti-Psm antibody
Anti-Psma antibody
Anti-Pteroylpoly-Gamma-Glutamate Carboxypeptidase antibody
Anti-FOLH1 antibody
Anti-FOLH antibody
Anti-NAALAD1 antibody
Anti-PSM antibody
Anti-PSMA antibody
Anti-GIG27 antibody

Information sourced from Uniprot.org

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