• Confocal imaging of paraffin-embedded Rat liver tissue using FMO3 Rabbit monoclonal antibody (STJ11102955, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Human liver tissue using FMO3 Rabbit monoclonal antibody (STJ11102955, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse liver tissue using FMO3 Rabbit monoclonal antibody (STJ11102955, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using FMO3 Rabbit monoclonal antibody (STJ11102955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using FMO3 Rabbit monoclonal antibody (STJ11102955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FMO3 antibody (300-400) [S5MR] (STJ11102955)

SKU:
STJ11102955

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FMO3 (300-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FMO3
Gene ID: 2328
Uniprot ID: FMO3_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human FMO3 (P31513).
Immunogen Sequence: KPNVKEFTETSAIFEDGTIF EGIDCVIFATGYSFAYPFLD ESIIKSRNNEIILFKGVFPP LLEKSTIAVIGFVQSLGAAI PTVDLQSRWAAQVIKGTCTL P
Tissue Specificity Liver.
Function Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds. Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation.
Protein Name Flavin-Containing Monooxygenase 3
Dimethylaniline Monooxygenase N-Oxide-Forming 3
Dimethylaniline Oxidase 3
Fmo Ii
Fmo Form 2
Hepatic Flavin-Containing Monooxygenase 3
Fmo 3
Trimethylamine Monooxygenase
Database Links Reactome: R-HSA-217271
Reactome: R-HSA-5579019
Cellular Localisation Microsome Membrane
Single-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Flavin-Containing Monooxygenase 3 antibody
Anti-Dimethylaniline Monooxygenase N-Oxide-Forming 3 antibody
Anti-Dimethylaniline Oxidase 3 antibody
Anti-Fmo Ii antibody
Anti-Fmo Form 2 antibody
Anti-Hepatic Flavin-Containing Monooxygenase 3 antibody
Anti-Fmo 3 antibody
Anti-Trimethylamine Monooxygenase antibody
Anti-FMO3 antibody

Information sourced from Uniprot.org

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