| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FLOT1 |
| Gene ID: |
10211 |
| Uniprot ID: |
FLOT1_HUMAN |
| Immunogen Region: |
155-427 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 155-427 of human Flotillin 1 (NP_005794.1). |
| Immunogen Sequence: |
HDDQDYLHSLGKARTAQVQK DARIGEAEAKRDAGIREAKA KQEKVSAQYLSEIEMAKAQR DYELKKAAYDIEVNTRRAQA DLAYQLQVAKTKQQIEEQRV QVQVVERAQQVAVQEQEIAR REKELEARVRKPAEAERYKL ERLAEAEKSQLIMQAEAEAA SVRMRGEAEAFAIGARARAE AEQMAKKAEAFQLYQEAAQL DMLLEKLPQVAEEISGPLTS ANKITLVSSGSGTMGAAKV |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389175/STJ27976_3__74293.1713158812.jpg?c=1 "Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389176/STJ27976_4__36753.1713158813.jpg?c=1 "Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389177/STJ27976_5__16396.1713158814.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101432/389178/STJ27976_6__26072.1713158814.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit polyclonal antibody (STJ27976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
