| Host: | Mouse |
| Applications: | WB/ELISA/IP/IF/IP |
| Reactivity: | Species independent |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Flag-Tag-Tag is suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 12M11 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS pH7.4, containing 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Purification: | Protein A purified. |
| Dilution Range: | WB 1:10000-50000ELISA 1:20000-50000IP 1:50-1:200IF 1:1000-3000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Specificity: | This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i |
| Immunogen: | Synthetic Peptide of Flag-Tag |
| Background | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
Information sourced from Uniprot.org
