• RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Zhihua Liu WB, IP Human 1:5000 HEK 293 T cell, KYSE30 cell, KYSE450 cell
  • Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody (STJS000791) was diluted at 1:10000
  • Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody (catalog#:STJ99665) was diluted at 1:10000
  • 1) Input: 293T transfected N-flag protein lysate 2) IP product: IP dilute 1: 200, 293T transfected N-flag protein lysateWestern blot analysis: primary antibody : 1:10000 Secondary antibody: Goat anti- mouse IgG (STJ99665), 1: 5000
  • Immunofluorescence analysis of 293T transfected FLAG and GFP tag protein. 1, FLAG primary Antibody (red STJA0006221) was diluted at 1:1000 (4°C overnight), GFP primary Antibody (green YN5582) was diluted at 1:1000 (4°C overnight) ,

Anti-Flag-Tag antibody [12M11] (STJA0006221)

SKU:
STJA0006221

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/ELISA/IP/IF/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Flag-Tag-Tag is suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 12M11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, containing 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: Protein A purified.
Dilution Range: WB 1:10000-50000
ELISA 1:20000-50000
IP 1:50-1:200
IF 1:1000
-3000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i
Immunogen: Synthetic Peptide of Flag-Tag
Background The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance