| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | FKBP10 |
| Gene ID: | 60681 |
| Uniprot ID: | FKB10_HUMAN |
| Immunogen Region: | 303-582 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human FKBP10 (NP_068758.3). |
| Immunogen Sequence: | FDSSYSRNHTYNTYIGQGYI IPGMDQGLQGACMGERRRIT IPPHLAYGENGTGDKIPGSA VLIFNVHVIDFHNPADVVEI RTLSRPSETCNETTKLGDFV RYHYNCSLLDGTQLFTSHDY GAPQEATLGANKVIEGLDTG LQGMCVGERRQLIVPPHLAH GESGARGVPGSAVLLFEVEL VSREDGLPTGYLFVWHKDPP ANLFEDMDLNKDGEVPPEEF STFIKAQVSEGKGRLMPGQ |
| Post Translational Modifications | Glycosylated and phosphorylated. |
| Function | PPIases accelerate the folding of proteins during protein synthesis. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase Fkbp10Ppiase Fkbp1065 Kda Fk506-Binding Protein65 Kda FkbpFkbp-65Fk506-Binding Protein 10Fkbp-10Immunophilin Fkbp65Rotamase |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Peptidyl-Prolyl Cis-Trans Isomerase Fkbp10 antibodyAnti-Ppiase Fkbp10 antibodyAnti-65 Kda Fk506-Binding Protein antibodyAnti-65 Kda Fkbp antibodyAnti-Fkbp-65 antibodyAnti-Fk506-Binding Protein 10 antibodyAnti-Fkbp-10 antibodyAnti-Immunophilin Fkbp65 antibodyAnti-Rotamase antibodyAnti-FKBP10 antibodyAnti-FKBP65 antibodyAnti-PSEC0056 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) 293T cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ11100746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89684/358175/STJ11100746_1__66574.1713122483.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) 293T cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ11100746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.")
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/93884/375089/STJ114797_1__72218.1713141770.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
