• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g TTC11/FIS1 antibody (STJ11101755). Western blot was performed from the immunoprecipitate using TTC11/FIS1 (STJ11101755) at a dilution of 1:2000.
  • Confocal imaging of U-2 OS cells using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of TTC11/FIS1 in paraffin-embedded human colon carcinoma using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TTC11/FIS1 in paraffin-embedded rat brain using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from wild type (WT) and TTC11/FIS1 knockout (KO) HeLa (KO) cells, using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FIS1 antibody (1-122) [S5MR] (STJ11101755)

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STJ11101755

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TTC11 (1-122) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FIS1
Gene ID: 51024
Uniprot ID: FIS1_HUMAN
Immunogen Region: 1-122
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11/FIS1 (NP_057152.2).
Immunogen Sequence: MEAVLNELVSVEDLLKFEKK FQSEKAAGSVSKSTQFEYAW CLVRSKYNDDIRKGIVLLEE LLPKGSKEEQRDYVFYLAVG NYRLKEYEKALKYVRGLLQT EPQNNQAKELERLIDKAMKK DG
Post Translational Modifications Ubiquitinated by MARCHF5.
Function Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event. Also mediates peroxisomal fission. May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis.
Protein Name Mitochondrial Fission 1 Protein
Fis1 Homolog
Hfis1
Tetratricopeptide Repeat Protein 11
Tpr Repeat Protein 11
Database Links Reactome: R-HSA-9603798
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Peroxisome Membrane
Alternative Antibody Names Anti-Mitochondrial Fission 1 Protein antibody
Anti-Fis1 Homolog antibody
Anti-Hfis1 antibody
Anti-Tetratricopeptide Repeat Protein 11 antibody
Anti-Tpr Repeat Protein 11 antibody
Anti-FIS1 antibody
Anti-TTC11 antibody
Anti-CGI-135 antibody

Information sourced from Uniprot.org

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