![Confocal imaging of U-2 OS cells using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90670/360678/STJ11101755_2__74282.1713125373.jpg?c=1 "Confocal imaging of U-2 OS cells using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.")
![Immunohistochemistry analysis of TTC11/FIS1 in paraffin-embedded human colon carcinoma using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90670/360679/STJ11101755_3__74254.1713125375.jpg?c=1 "Immunohistochemistry analysis of TTC11/FIS1 in paraffin-embedded human colon carcinoma using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of TTC11/FIS1 in paraffin-embedded rat brain using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90670/360680/STJ11101755_4__05669.1713125376.jpg?c=1 "Immunohistochemistry analysis of TTC11/FIS1 in paraffin-embedded rat brain using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of various lysates, using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90670/360681/STJ11101755_5__86439.1713125377.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and TTC11/FIS1 knockout (KO) HeLa (KO) cells, using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90670/360682/STJ11101755_6__32734.1713125377.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TTC11/FIS1 knockout (KO) HeLa (KO) cells, using [KO Validated] TTC11/FIS1 Rabbit monoclonal antibody (STJ11101755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IHC-P:1:200-1:800 IF/ICC:1:100-1:400 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your spe |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FIS1 |
| Gene ID: | 51024 |
| Uniprot ID: | FIS1_HUMAN |
| Immunogen Region: | 1-122 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11/FIS1 (NP_057152.2). |
| Immunogen Sequence: | MEAVLNELVSVEDLLKFEKK FQSEKAAGSVSKSTQFEYAW CLVRSKYNDDIRKGIVLLEE LLPKGSKEEQRDYVFYLAVG NYRLKEYEKALKYVRGLLQT EPQNNQAKELERLIDKAMKK DG |
| Post Translational Modifications | Ubiquitinated by MARCHF5. |
| Function | Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event. Also mediates peroxisomal fission. May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. |
| Protein Name | Mitochondrial Fission 1 Protein Fis1 Homolog Hfis1 Tetratricopeptide Repeat Protein 11 Tpr Repeat Protein 11 |
| Database Links | Reactome: R-HSA-9603798 |
| Cellular Localisation | Mitochondrion Outer Membrane Single-Pass Membrane Protein Peroxisome Membrane |
| Alternative Antibody Names | Anti-Mitochondrial Fission 1 Protein antibody Anti-Fis1 Homolog antibody Anti-Hfis1 antibody Anti-Tetratricopeptide Repeat Protein 11 antibody Anti-Tpr Repeat Protein 11 antibody Anti-FIS1 antibody Anti-TTC11 antibody Anti-CGI-135 antibody |
Information sourced from Uniprot.org