![Western blot analysis of lysates from wild type (WT) and FIBP knockout (KO) HeLa cells, using [KO Validated] FIBP Rabbit polyclonal antibody (STJ11100784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89720/358201/STJ11100784_1__82816.1713122521.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FIBP knockout (KO) HeLa cells, using [KO Validated] FIBP Rabbit polyclonal antibody (STJ11100784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and FIBP knockout (KO) HeLa cells, using [KO Validated] FIBP Rabbit polyclonal antibody (STJ11100784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89720/358201/STJ11100784_1__82816.1713122521.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FIBP knockout (KO) HeLa cells, using [KO Validated] FIBP Rabbit polyclonal antibody (STJ11100784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FIBP |
| Gene ID: | 9158 |
| Uniprot ID: | FIBP_HUMAN |
| Immunogen Region: | 1-364 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human FIBP (NP_004205.2). |
| Immunogen Sequence: | MTSELDIFVGNTTLIDEDVY RLWLDGYSVTDAVALRVRSG ILEQTGATAAVLQSDTMDHY RTFHMLERLLHAPPKLLHQL IFQIPPSRQALLIERYYAFD EAFVREVLGKKLSKGTKKDL DDISTKTGITLKSCRRQFDN FKRVFKVVEEMRGSLVDNIQ QHFLLSDRLARDYAAIVFFA NNRFETGKKKLQYLSFGDFA FCAELMIQNWTLGAVDSQMD DMDMDLDKEFLQDLKELKV |
| Tissue Specificity | Highly expressed in heart, skeletal muscle and pancreas. Expressed at lower levels in brain. Also found in placenta, liver and kidney. |
| Function | May be involved in mitogenic function of FGF1. May mediate with IER2 FGF-signaling in the establishment of laterality in the embryo. |
| Protein Name | Acidic Fibroblast Growth Factor Intracellular-Binding ProteinAfgf Intracellular-Binding ProteinFgf-1 Intracellular-Binding Protein |
| Cellular Localisation | NucleusEndomembrane SystemPeripheral Membrane ProteinAlso Associated With Cytoplasmic MembranesParticularly Of Mitochondria |
| Alternative Antibody Names | Anti-Acidic Fibroblast Growth Factor Intracellular-Binding Protein antibodyAnti-Afgf Intracellular-Binding Protein antibodyAnti-Fgf-1 Intracellular-Binding Protein antibodyAnti-FIBP antibody |
Information sourced from Uniprot.org