| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-FHL1/SLIM1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 32kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates and in Rat Lung and Testis lysates (calculated MW of 31.9kDa according to Mouse NP_001274729.1 and Rat NP_001 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | FHL1 |
| Gene ID: | 2273 |
| Uniprot ID: | FHL1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001440.2; NP_001153171.1 |
| Specificity: | This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variant |
| Immunogen Sequence: | CNKRFVFHNEQVY |
| Function | May have an involvement in muscle development or hypertrophy. |
| Protein Name | Four And A Half Lim Domains Protein 1Fhl-1Skeletal Muscle Lim-Protein 1SlimSlim-1 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 3: CytoplasmNucleusIsoform 2: NucleusCytoplasmCytosolPredominantly Nuclear In Myoblasts But Is Cytosolic In Differentiated Myotubes |
| Alternative Antibody Names | Anti-Four And A Half Lim Domains Protein 1 antibodyAnti-Fhl-1 antibodyAnti-Skeletal Muscle Lim-Protein 1 antibodyAnti-Slim antibodyAnti-Slim-1 antibodyAnti-FHL1 antibodyAnti-SLIM1 antibody |
Information sourced from Uniprot.org