| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-FGFR1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 75kDa band observed in Human Breast lysates (calculated MW of 82.2kDa according to NP_075593, 81.9kDa according to NP_075594). Recommended concentration: 0.3-1µg/ml. IHC: In |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | FGFR1 |
| Gene ID: | 2260 |
| Uniprot ID: | FGFR1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1 |
| Specificity: | This antibody is expected to recognise five isoforms (as represented by NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1). |
| Immunogen Sequence: | CLPRHPAQLANGGLKR |
| Post Translational Modifications | Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation. Ubiquitinated. FGFR1 is rapidly ubiquitinated by NEDD4 after autophosphorylation, leading to internalization and lysosomal degradation. CBL is recruited to activated FGFR1 via FRS2 and GRB2, and mediates ubiquitination and subsequent degradation of FGFR1. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. |
| Protein Name | Fibroblast Growth Factor Receptor 1 Fgfr-1 Basic Fibroblast Growth Factor Receptor 1 Bfgfr Bfgf-R-1 Fms-Like Tyrosine Kinase 2 Flt-2 N-Sam Proto-Oncogene C-Fgr Cd Antigen Cd331 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839120 Reactome: R-HSA-1839122 Reactome: R-HSA-190370 P11362-19 Reactome: R-HSA-190373 P11362-1 Reactome: R-HSA-190374 P11362-1 Reactome: R-HSA-2219530 Reactome: R-HSA-375165 P11362-1 Reactome: R-HSA-445144 P11362-1 Reactome: R-HSA-5654219 Reactome: R-HSA-5654687 Reactome: R-HSA-5654688 Reactome: R-HSA-5654689 Reactome: R-HSA-5654693 Reactome: R-HSA-5654726 Reactome: R-HSA-5655302 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8853336 Reactome: R-HSA-9758919 Reactome: R-HSA-9793380 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Nucleus Cytoplasm Cytosol Cytoplasmic Vesicle After Ligand Binding Both Receptor And Ligand Are Rapidly Internalized Can Translocate To The Nucleus After Internalization Or By Translocation From The Endoplasmic Reticulum Or Golgi Apparatus To The Cytosol And From There To The Nucleus |
| Alternative Antibody Names | Anti-Fibroblast Growth Factor Receptor 1 antibody Anti-Fgfr-1 antibody Anti-Basic Fibroblast Growth Factor Receptor 1 antibody Anti-Bfgfr antibody Anti-Bfgf-R-1 antibody Anti-Fms-Like Tyrosine Kinase 2 antibody Anti-Flt-2 antibody Anti-N-Sam antibody Anti-Proto-Oncogene C-Fgr antibody Anti-Cd Antigen Cd331 antibody Anti-FGFR1 antibody Anti-BFGFR antibody Anti-CEK antibody Anti-FGFBR antibody Anti-FLG antibody Anti-FLT2 antibody Anti-HBGFR antibody |
Information sourced from Uniprot.org
