Anti-FBXO7 antibody (353-522) (STJ29600)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FBXO7 (353-522) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXO7 |
| Gene ID | 25793 |
| Uniprot ID | FBX7_HUMAN |
| Immunogen Region | 353-522 |
| Immunogen Sequence | LSLSAVCRDLFTASNDPLLW RFLYLRDFRDNTVRVQDTDW KELYRKRHIQRKESPKGRFV MLLPSSTHTIPFYPNPLHPR PFPSSRLPPGIIGGEYDQRP TLPYVGDPISSLIPGPGETP SQFPPLRPRFDPVGPLPGPN PILPGRGGPNDRFPFRPSRG RPTDGRLSFM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 353-522 of human FBXO7 (NP_036311.3). |
Additional Info
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability. Recognizes and ubiquitinates BIRC2 and the cell cycle regulator DLGAP5. Plays a role downstream of PINK1 in the clearance of damaged mitochondria via selective autophagy (mitophagy) by targeting PRKN to dysfunctional depolarized mitochondria. Promotes MFN1 ubiquitination. Mediates the ubiquitination and proteasomal degradation of UXT isoform 2, thereby impairing the NF-kappa-B signaling pathway. Inhibits NF-kappa-B pathway also by promoting the ubiquitination of TRAF2. Affects the assembly state and activity of the proteasome in the cells including neurons by ubiquitinating the proteasomal subunit PSMA2 via 'Lys-63'-linked polyubiquitin chains. Promotes 'Lys-48'-linked polyubiquitination SIRT7, leading to the hydrogen peroxide-induced cell death. |
| Protein Name | F-Box Only Protein 7 |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusMitochondrionCytosolPredominantly CytoplasmicA Minor Proportion Is Detected In The NucleusRelocates From The Cytosol To Depolarized Mitochondria |
| Alternative Antibody Names | Anti-F-Box Only Protein 7 antibodyAnti-FBXO7 antibodyAnti-FBX7 antibody |
Information sourced from Uniprot.org