| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Cow/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-FBXO32 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 40kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 41.5kDa according to Mouse NP_080622.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | FBXO32 |
| Gene ID: | 114907 |
| Uniprot ID: | FBX32_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_478136.1 |
| Specificity: | This antibody is expected to recognize reported isoform 1 (NP_478136.1). |
| Immunogen Sequence: | NSKTKTQYFHQEK |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. |
| Protein Name | F-Box Only Protein 32Atrogin-1Muscle Atrophy F-Box ProteinMafbx |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-9615017Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusShuttles Between Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-F-Box Only Protein 32 antibodyAnti-Atrogin-1 antibodyAnti-Muscle Atrophy F-Box Protein antibodyAnti-Mafbx antibodyAnti-FBXO32 antibody |
Information sourced from Uniprot.org
