Western blot analysis of various lysates using FBXO2 Rabbit pAb (STJ111312) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-FBXO2 antibody (1-296) (STJ111312)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111312
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General Information

Short DescriptionRabbit polyclonal FBXO2 (1-296) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolFBXO2
Gene ID26232
Uniprot IDFBX2_HUMAN
Immunogen Region1-296
Immunogen SequenceMDGDGDPESVGQPEEASPEE QPEEASAEEERPEDQQEEEA AAAAAYLDELPEPLLLRVLA ALPAAELVQACRLVCLRWKE LVDGAPLWLLKCQQEGLVPE GGVEEERDHWQQFYFLSKRR RNLLRNPCGEEDLEGWCDVE HGGDGWRVEELPGDSGVEFT HDESVKKYFASSFEWCRKAQ VIDLQAEGYWEELLDTTQPA IVVKDWYSGRSDAGCLYELT VKLLSEHENVLAEFSSGQV
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human FBXO2 (NP_036300.2).

Additional Info

Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type.
Protein Name F-Box Only Protein 2
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Microsome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-F-Box Only Protein 2 antibody
Anti-FBXO2 antibody
Anti-FBX2 antibody

Information sourced from Uniprot.org

Citations

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