• Western blot analysis of extracts of various cell lines, using FBXO2 antibody (STJ111312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using FBXO2 antibody (STJ111312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FBXO2 antibody (1-296) (STJ111312)

SKU:
STJ111312

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBXO2 (1-296) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO2
Gene ID: 26232
Uniprot ID: FBX2_HUMAN
Immunogen Region: 1-296
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human FBXO2 (NP_036300.2).
Immunogen Sequence: MDGDGDPESVGQPEEASPEE QPEEASAEEERPEDQQEEEA AAAAAYLDELPEPLLLRVLA ALPAAELVQACRLVCLRWKE LVDGAPLWLLKCQQEGLVPE GGVEEERDHWQQFYFLSKRR RNLLRNPCGEEDLEGWCDVE HGGDGWRVEELPGDSGVEFT HDESVKKYFASSFEWCRKAQ VIDLQAEGYWEELLDTTQPA IVVKDWYSGRSDAGCLYELT VKLLSEHENVLAEFSSGQV
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type.
Protein Name F-Box Only Protein 2
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Microsome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-F-Box Only Protein 2 antibody
Anti-FBXO2 antibody
Anti-FBX2 antibody

Information sourced from Uniprot.org

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