Anti-FBXO11 antibody (754-765) (STJ73551)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ73551-100
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General Information

Short DescriptionGoat polyclonal antibody anti-FBXO11 (754-765) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Pig/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 90kDa band observed in nuclear lysates of cell line HeLa and lysates of cell line A431 (calculated MW of 94.1kDa according to NP_079409.3). Recommended concentration: 0.1-0.3µg/
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolFBXO11
Gene ID80204
Uniprot IDFBX11_HUMAN
Accession NumberNP_079409.3; NP_001177203.1; XP_005264629.1; XP_005264630.1; XP_005264631.1; XP_005264632.1
Immunogen Region754-765
Immunogen SequenceKISSYTSYPMHD
SpecificityThis antibody is expected to recognize all reported isoforms (NP_079409.3; NP_001177203.1; XP_005264629.1; XP_005264630.1; XP_005264631.1; XP_005264632.1).

Additional Info

Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6, SNAI1 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit. The SCF(FBXO11) complex also catalyzes ubiquitination and degradation of GSK3B-phosphorylated SNAI1. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. Plays a role in the regulatiom of erythropoiesis but not myelopoiesis or megakaryopoiesis. Mechanistically, activates erythroid genes by mediating the degradation of BAHD1, a heterochromatin-associated protein that recruits corepressors to H3K27me3 marks. Participates in macrophage cell death and inflammation in response to bacterial toxins by regulating the expression of complement 5a receptor 1/C5AR1 and IL-1beta. Acts as a critical regulator to determine the level of MHC-II by mediating the recognition of degron at the P/S/T domain of CIITA leading to its ubiquitination and subsequent degradation via the proteasome. Participates in the antiviral repsonse by initiating the activation of TBK1-IRF3-IFN-I axis. Mediates the 'Lys-63'-linked ubiquitination of TRAF3 to strengthen the interaction between TRAF3 and TBK1.
Protein Name F-Box Only Protein 11
Protein Arginine N-Methyltransferase 9
Vitiligo-Associated Protein 1
Vit-1
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-F-Box Only Protein 11 antibody
Anti-Protein Arginine N-Methyltransferase 9 antibody
Anti-Vitiligo-Associated Protein 1 antibody
Anti-Vit-1 antibody
Anti-FBXO11 antibody
Anti-FBX11 antibody
Anti-PRMT9 antibody
Anti-VIT1 antibody
Anti-UG063H01 antibody

Information sourced from Uniprot.org

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