• Western blot analysis of extracts of various cell lines, using FBLN5 antibody (STJ112002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of L929 cells using FBLN5 Polyclonal Antibody (STJ112002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of A-431 cells, using FBLN5 antibody (STJ112002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of A-431 cells, using FBLN5 antibody (STJ112002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of L929 cells using FBLN5 Polyclonal Antibody (STJ112002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FBLN5 antibody (STJ112002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FBLN5 antibody (199-448) (STJ112002)

SKU:
STJ112002

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBLN5 (199-448) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBLN5
Gene ID: 10516
Uniprot ID: FBLN5_HUMAN
Immunogen Region: 199-448
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 199-448 of human FBLN5 (NP_006320.2).
Immunogen Sequence: NEDGRSCQDVNECATENPCV QTCVNTYGSFICRCDPGYEL EEDGVHCSDMDECSFSEFLC QHECVNQPGTYFCSCPPGYI LLDDNRSCQDINECEHRNHT CNLQQTCYNLQGGFKCIDPI RCEEPYLRISDNRCMCPAEN PGCRDQPFTILYRDMDVVSG RSVPADIFQMQATTRYPGAY YIFQIKSGNEGREFYMRQTG PISATLVMTRPIKGPREIQL DLEMITVNTVINFRGSSVI
Tissue Specificity Expressed in skin fibroblasts (at protein level). Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.
Post Translational Modifications N-glycosylated.
Function Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. May act as an adapter that mediates the interaction between FBN1 and ELN.
Protein Name Fibulin-5
Fibl-5
Developmental Arteries And Neural Crest Egf-Like Protein
Dance
Urine P50 Protein
Up50
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-2129379
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Co-Localizes With Eln In Elastic Fibers
Alternative Antibody Names Anti-Fibulin-5 antibody
Anti-Fibl-5 antibody
Anti-Developmental Arteries And Neural Crest Egf-Like Protein antibody
Anti-Dance antibody
Anti-Urine P50 Protein antibody
Anti-Up50 antibody
Anti-FBLN5 antibody
Anti-DANCE antibody
Anti-UNQ184 antibody
Anti-PRO210 antibody

Information sourced from Uniprot.org

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